Synthetic Primers for Diseases Diagnostics

1. Selection of the target sequence and designing specific primer sets: Our team of experts use their knowledge and expertise to select the most appropriate target sequence, and design primer sets specific to the target sequence.
2. Bioinformatics analysis: Bioinformatics is done to guarantee that the target sequence will be amplified effectively and precisely by the primers.
3. Synthetic primer synthesis: We use cutting-edge chemistry to synthesize the primers after they have been created and validated in In-silico. Modern Oligo synthesis techniques guarantee that the primers are high quality and efficient.
4. In-vitro analysis: Laboratory experimental validation of designed primers through PCR amplification testing is the final step in our primer design and optimization process. We use different downstream applications, such as PCR, RT-qPCR, NGS, etc. to validate the efficiency and specificity of our primer sets. The testing also involves optimizing PCR conditions, such as reagent concentrations and annealing temperatures. The testing ensures amplification succeeds and avoids primer dimers and PCR chimera formation.

The importance of using high-quality primers for downstream applications is gaining traction. Therefore, we chose multi-step experimental processes so that our synthetic primers are of the highest quality and validated for various new-generation molecular biology assays.