Description
| Flu SC2 Multiplex Assay: Primers | |||
| Name | Description | Oligonucleotide Sequence (5’ to 3’) | Concentration |
| InfA-F | InfA For1 | 5’-CAA GAC CAA TCY TGT CAC CTC TGA C-3’ | 100 mM |
| InfA For2 | 5’-CAA GAC CAA TYC TGT CAC CTY TGA C-3’ | 100 mM | |
| InfA-R | InfA Rev1 | 5’-GCA TTY TGG ACA AAV CGT CTA CG-3’ | 100 mM |
| InfA Rev2 | 5’-GCA TTT TGG ATA AAG CGT CTA CG-3’ | 100 mM | |
| InfB-F | InfB For | 5’-TCC TCA AYT CAC TCT TCG AGC G-3’ | 100 mM |
| InfB-R | InfB Rev | 5’-CGG TGC TCT TGA CCA AAT TGG-3’ | 100 mM |
| SC2-F | SC2 For | 5’-CTG CAG ATT TGG ATG ATT TCT CC-3’ | 100 mM |
| SC2-R | SC2 Rev | 5’-CCT TGT GTG GTC TGC ATG AGT TTA G-3’ | 100 mM |
| RP-F | RNase P For | 5’-AGA TTT GGA CCT GCG AGC G-3’ | 100 mM |
| RP-R | RNase P Rev | 5’-GAG CGG CTG TCT CCA CAA GT-3’ | 100 mM |
| Flu SC2 Multiplex Assay: Probes | |||
| Name | Description | Oligonucleotide Sequence (5’>3’) | Concentration |
| Option 1: Probes | |||
| InfA-P | InfA Probe | 5’-/FAM/TGC AGT CCT /ZEN/CGC TCA CTG GGC ACG/3IABkFQ/-3’ | 100 mM |
| InfB-P | InfB Probe | 5’-/YakYel/CCA ATT CGA /ZEN/ GCA GCT GAA ACT GCG GTG/3IABkFQ/-3’ | 100 mM |
| SC2-P | SC2 Probe | 5’-/TexRd-XN/ATT GCA ACA /TAO/ ATC CAT GAG CAG TGC TGA CTC/3IAbRQSp/-3’ | 100 mM |
| RP-P | RNase P Probe | 5’-/CY5/TTC TGA CCT /TAO/ GAA GGC TCT GCG CG/3IAbRQSp/-3’ | 100 mM |
| Option 2: Probes | |||
| InfA-P | InfA Probe | 5’-/FAM/TGC AGT CCT /Nova/ CGC TCA CTG GGC ACG/BHQ-1/-3’ | 100 mM |
| InfB-P | InfB Probe | 5’-/CIV-550/CCA ATT CGA /BHQ-1/ GCA GCT GAA ACT GCG GTG/C3/-3’ | 100 mM |
| SC2-P | SC2 Probe | 5’-/CalFluor610/ATT GCA ACA /Nova/ ATC CAT GAG CAG TGC TGA CTC/BHQ-2/-3’ | 100 mM |
| RP-P | RNase P Probe | 5’-/Quasar670/TTC TGA CCT /Nova/ GAA GGC TCT GCG CG/BHQ-2/-3’ | 100 mM |
